In situ Technologies

Recently, the most common techniques of environmental burden removal with an excellent performance in EPS biotechnology.

In complex site conditions, classic remediation methods may become insufficient or economically unfeasible. Therefore, we often supplement them with novel in situ techniques and provide tailor - made solutions for specific site conditions.
Making the most of biostimulation and lateral gene transfer: Along with nutrients and terminal electron acceptors (oxygen, nitrates, etc.), this technique introduces allochthonous microorganisms with the target pollutant affinity (cultured from the EPS biodegrading strain library) into the contaminated subsurface.
Simplified sketch of the bioaugmentation technology application
Inviting the autochthonous microorganisms to a ball and boosting their pollutant degrading metabolism through the injection of nutrient (N & P) and electron acceptor (O2, NO3, SO4, or Fe2+ and Mn2+) solutions.
Aka In situ chemical oxidation is the physico-chemical way of contaminant destruction/mineralization by direct oxidant exposure. One of the strongest remediation methods often combined with classic techniques such as pump and treat, venting, or soil washing.
Aka In situ chemical reduction is commonly used for chlorinated hydrocarbons’ (PCE, TCE, DCE, VC) de-chlorination. Infiltration of reductive agents (lactates, zero valent iron, thiosulfates, etc.) supplies the environment with electrons necessary for the job in hand.
Soil Washing (surfactants)
Enhancing the pollutant desorption: 1) higher surfactant concentrations to release the contaminant free phase for pumping, or 2) lower surfactant concentration to enhance the contaminant bioavailability by its gradual release into groundwater.
In another words, blowing the air into the aquifer, at least 2 m below the groundwater table: 1) To mechanically mobilize the free phase pollutants (NAPLs) and 2) To supply the terminal electron acceptor (O2) for aerobic bioremediation processes.
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Czech Republic
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